λ protein phosphatase Search Results


calmodulin affinity resins  (New England Biolabs)
98
New England Biolabs calmodulin affinity resins
Calmodulin Affinity Resins, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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calmodulin affinity resins - by Bioz Stars, 2026-08
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λ protein phosphatase 100u  (New England Biolabs)
98
New England Biolabs λ protein phosphatase 100u
λ Protein Phosphatase 100u, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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λ protein phosphatase 100u - by Bioz Stars, 2026-08
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λ protein phosphatase  (Merck KGaA)
90
Merck KGaA λ protein phosphatase
Analysis of Pkc1 electrophoretic mobility after induction of DNA damage. ( A ) Left panel: exponentially growing wild-type cells expressing a GFP-tagged Pkc1 protein (JCY1511) were incubated for 2 h in the absence or presence of 0.2 M HU; right panel: raffinose-grown wild-type (JCY1511) and GAL1:HO (JCY1514) cells expressing a GFP-tagged Pkc1 protein were incubated for 4 h in the presence of 2% glucose or 2% galactose. Electrophoretic mobility of Pkc1-GFP was investigated by western analysis using an anti-GFP antibody. An unspecific band in the western blot or the ponceau staining of the membrane is showed as a control of migration. Activation of the checkpoint kinase Rad53 is showed as a control of DNA damage induction. ( B ) Raffinose-grown GAL1:HO (JKM139) cells were incubated for 4 h in the presence of 2% glucose or 2% galactose. Electrophoretic migration of endogenous Pkc1 was investigated by western analysis using an anti-yeast Pkc1 antibody. ( C ) Raffinose-grown GAL1:HO (JKM139) and GAL1:HO tel1 (JCY1654) cells were incubated for 4 h in the presence of 2% glucose or 2% galactose. Electrophoretic migration of endogenous Pkc1 after incubation of cell extracts in the absence or presence of <t>λ</t> <t>protein</t> <t>phosphatase</t> was analyzed as in (B). ( D ) Electrophoretic migration of Pkc1-GFP in wild-type (W303-1a) mec1 (JCY1580) and tel1 (JCY1583) cells after addition of HU was analyzed as described in (A). ( E ) Raffinose-grown GAL1:HO (JKM139) and GAL1:HO tel1 (JCY1654) cells transformed with pPKC1-GFP plasmid were incubated for 4 h in the presence of 2% glucose or 2% galactose. Electrophoretic migration of Pkc1-GFP was investigated by western analysis using an anti-GFP antibody.
λ Protein Phosphatase, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/%CE%BB+protein+phosphatase/pmc04066786-88-38-41?v=Merck+KGaA
Average 90 stars, based on 1 article reviews
λ protein phosphatase - by Bioz Stars, 2026-08
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lambda protein phosphatase  (ApexBio)
90
ApexBio lambda protein phosphatase
Analysis of Pkc1 electrophoretic mobility after induction of DNA damage. ( A ) Left panel: exponentially growing wild-type cells expressing a GFP-tagged Pkc1 protein (JCY1511) were incubated for 2 h in the absence or presence of 0.2 M HU; right panel: raffinose-grown wild-type (JCY1511) and GAL1:HO (JCY1514) cells expressing a GFP-tagged Pkc1 protein were incubated for 4 h in the presence of 2% glucose or 2% galactose. Electrophoretic mobility of Pkc1-GFP was investigated by western analysis using an anti-GFP antibody. An unspecific band in the western blot or the ponceau staining of the membrane is showed as a control of migration. Activation of the checkpoint kinase Rad53 is showed as a control of DNA damage induction. ( B ) Raffinose-grown GAL1:HO (JKM139) cells were incubated for 4 h in the presence of 2% glucose or 2% galactose. Electrophoretic migration of endogenous Pkc1 was investigated by western analysis using an anti-yeast Pkc1 antibody. ( C ) Raffinose-grown GAL1:HO (JKM139) and GAL1:HO tel1 (JCY1654) cells were incubated for 4 h in the presence of 2% glucose or 2% galactose. Electrophoretic migration of endogenous Pkc1 after incubation of cell extracts in the absence or presence of <t>λ</t> <t>protein</t> <t>phosphatase</t> was analyzed as in (B). ( D ) Electrophoretic migration of Pkc1-GFP in wild-type (W303-1a) mec1 (JCY1580) and tel1 (JCY1583) cells after addition of HU was analyzed as described in (A). ( E ) Raffinose-grown GAL1:HO (JKM139) and GAL1:HO tel1 (JCY1654) cells transformed with pPKC1-GFP plasmid were incubated for 4 h in the presence of 2% glucose or 2% galactose. Electrophoretic migration of Pkc1-GFP was investigated by western analysis using an anti-GFP antibody.
Lambda Protein Phosphatase, supplied by ApexBio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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lambda protein phosphatase  (BioAcademia)
90
BioAcademia lambda protein phosphatase
Analysis of Pkc1 electrophoretic mobility after induction of DNA damage. ( A ) Left panel: exponentially growing wild-type cells expressing a GFP-tagged Pkc1 protein (JCY1511) were incubated for 2 h in the absence or presence of 0.2 M HU; right panel: raffinose-grown wild-type (JCY1511) and GAL1:HO (JCY1514) cells expressing a GFP-tagged Pkc1 protein were incubated for 4 h in the presence of 2% glucose or 2% galactose. Electrophoretic mobility of Pkc1-GFP was investigated by western analysis using an anti-GFP antibody. An unspecific band in the western blot or the ponceau staining of the membrane is showed as a control of migration. Activation of the checkpoint kinase Rad53 is showed as a control of DNA damage induction. ( B ) Raffinose-grown GAL1:HO (JKM139) cells were incubated for 4 h in the presence of 2% glucose or 2% galactose. Electrophoretic migration of endogenous Pkc1 was investigated by western analysis using an anti-yeast Pkc1 antibody. ( C ) Raffinose-grown GAL1:HO (JKM139) and GAL1:HO tel1 (JCY1654) cells were incubated for 4 h in the presence of 2% glucose or 2% galactose. Electrophoretic migration of endogenous Pkc1 after incubation of cell extracts in the absence or presence of <t>λ</t> <t>protein</t> <t>phosphatase</t> was analyzed as in (B). ( D ) Electrophoretic migration of Pkc1-GFP in wild-type (W303-1a) mec1 (JCY1580) and tel1 (JCY1583) cells after addition of HU was analyzed as described in (A). ( E ) Raffinose-grown GAL1:HO (JKM139) and GAL1:HO tel1 (JCY1654) cells transformed with pPKC1-GFP plasmid were incubated for 4 h in the presence of 2% glucose or 2% galactose. Electrophoretic migration of Pkc1-GFP was investigated by western analysis using an anti-GFP antibody.
Lambda Protein Phosphatase, supplied by BioAcademia, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
lambda protein phosphatase - by Bioz Stars, 2026-08
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λ protein phosphatase, 20,000 u  (Merck KGaA)
90
Merck KGaA λ protein phosphatase, 20,000 u
Analysis of Pkc1 electrophoretic mobility after induction of DNA damage. ( A ) Left panel: exponentially growing wild-type cells expressing a GFP-tagged Pkc1 protein (JCY1511) were incubated for 2 h in the absence or presence of 0.2 M HU; right panel: raffinose-grown wild-type (JCY1511) and GAL1:HO (JCY1514) cells expressing a GFP-tagged Pkc1 protein were incubated for 4 h in the presence of 2% glucose or 2% galactose. Electrophoretic mobility of Pkc1-GFP was investigated by western analysis using an anti-GFP antibody. An unspecific band in the western blot or the ponceau staining of the membrane is showed as a control of migration. Activation of the checkpoint kinase Rad53 is showed as a control of DNA damage induction. ( B ) Raffinose-grown GAL1:HO (JKM139) cells were incubated for 4 h in the presence of 2% glucose or 2% galactose. Electrophoretic migration of endogenous Pkc1 was investigated by western analysis using an anti-yeast Pkc1 antibody. ( C ) Raffinose-grown GAL1:HO (JKM139) and GAL1:HO tel1 (JCY1654) cells were incubated for 4 h in the presence of 2% glucose or 2% galactose. Electrophoretic migration of endogenous Pkc1 after incubation of cell extracts in the absence or presence of <t>λ</t> <t>protein</t> <t>phosphatase</t> was analyzed as in (B). ( D ) Electrophoretic migration of Pkc1-GFP in wild-type (W303-1a) mec1 (JCY1580) and tel1 (JCY1583) cells after addition of HU was analyzed as described in (A). ( E ) Raffinose-grown GAL1:HO (JKM139) and GAL1:HO tel1 (JCY1654) cells transformed with pPKC1-GFP plasmid were incubated for 4 h in the presence of 2% glucose or 2% galactose. Electrophoretic migration of Pkc1-GFP was investigated by western analysis using an anti-GFP antibody.
λ Protein Phosphatase, 20,000 U, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/%CE%BB+protein+phosphatase/10__1038_slash_nprot__2015__007-129-68-73?v=Merck+KGaA
Average 90 stars, based on 1 article reviews
λ protein phosphatase, 20,000 u - by Bioz Stars, 2026-08
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lambda protein phosphatase -ppase  (BioAcademia)
90
BioAcademia lambda protein phosphatase -ppase
Analysis of Pkc1 electrophoretic mobility after induction of DNA damage. ( A ) Left panel: exponentially growing wild-type cells expressing a GFP-tagged Pkc1 protein (JCY1511) were incubated for 2 h in the absence or presence of 0.2 M HU; right panel: raffinose-grown wild-type (JCY1511) and GAL1:HO (JCY1514) cells expressing a GFP-tagged Pkc1 protein were incubated for 4 h in the presence of 2% glucose or 2% galactose. Electrophoretic mobility of Pkc1-GFP was investigated by western analysis using an anti-GFP antibody. An unspecific band in the western blot or the ponceau staining of the membrane is showed as a control of migration. Activation of the checkpoint kinase Rad53 is showed as a control of DNA damage induction. ( B ) Raffinose-grown GAL1:HO (JKM139) cells were incubated for 4 h in the presence of 2% glucose or 2% galactose. Electrophoretic migration of endogenous Pkc1 was investigated by western analysis using an anti-yeast Pkc1 antibody. ( C ) Raffinose-grown GAL1:HO (JKM139) and GAL1:HO tel1 (JCY1654) cells were incubated for 4 h in the presence of 2% glucose or 2% galactose. Electrophoretic migration of endogenous Pkc1 after incubation of cell extracts in the absence or presence of <t>λ</t> <t>protein</t> <t>phosphatase</t> was analyzed as in (B). ( D ) Electrophoretic migration of Pkc1-GFP in wild-type (W303-1a) mec1 (JCY1580) and tel1 (JCY1583) cells after addition of HU was analyzed as described in (A). ( E ) Raffinose-grown GAL1:HO (JKM139) and GAL1:HO tel1 (JCY1654) cells transformed with pPKC1-GFP plasmid were incubated for 4 h in the presence of 2% glucose or 2% galactose. Electrophoretic migration of Pkc1-GFP was investigated by western analysis using an anti-GFP antibody.
Lambda Protein Phosphatase Ppase, supplied by BioAcademia, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/%CE%BB+protein+phosphatase/10__1523_slash_jneurosci__5261___13__2014-78-10-17?v=BioAcademia
Average 90 stars, based on 1 article reviews
lambda protein phosphatase -ppase - by Bioz Stars, 2026-08
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nini  (Sino Biological)
89
Sino Biological nini
Analysis of Pkc1 electrophoretic mobility after induction of DNA damage. ( A ) Left panel: exponentially growing wild-type cells expressing a GFP-tagged Pkc1 protein (JCY1511) were incubated for 2 h in the absence or presence of 0.2 M HU; right panel: raffinose-grown wild-type (JCY1511) and GAL1:HO (JCY1514) cells expressing a GFP-tagged Pkc1 protein were incubated for 4 h in the presence of 2% glucose or 2% galactose. Electrophoretic mobility of Pkc1-GFP was investigated by western analysis using an anti-GFP antibody. An unspecific band in the western blot or the ponceau staining of the membrane is showed as a control of migration. Activation of the checkpoint kinase Rad53 is showed as a control of DNA damage induction. ( B ) Raffinose-grown GAL1:HO (JKM139) cells were incubated for 4 h in the presence of 2% glucose or 2% galactose. Electrophoretic migration of endogenous Pkc1 was investigated by western analysis using an anti-yeast Pkc1 antibody. ( C ) Raffinose-grown GAL1:HO (JKM139) and GAL1:HO tel1 (JCY1654) cells were incubated for 4 h in the presence of 2% glucose or 2% galactose. Electrophoretic migration of endogenous Pkc1 after incubation of cell extracts in the absence or presence of <t>λ</t> <t>protein</t> <t>phosphatase</t> was analyzed as in (B). ( D ) Electrophoretic migration of Pkc1-GFP in wild-type (W303-1a) mec1 (JCY1580) and tel1 (JCY1583) cells after addition of HU was analyzed as described in (A). ( E ) Raffinose-grown GAL1:HO (JKM139) and GAL1:HO tel1 (JCY1654) cells transformed with pPKC1-GFP plasmid were incubated for 4 h in the presence of 2% glucose or 2% galactose. Electrophoretic migration of Pkc1-GFP was investigated by western analysis using an anti-GFP antibody.
Nini, supplied by Sino Biological, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 89 stars, based on 1 article reviews
nini - by Bioz Stars, 2026-08
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lambda protein phosphatase kit k1102  (ApexBio)
90
ApexBio lambda protein phosphatase kit k1102
Analysis of Pkc1 electrophoretic mobility after induction of DNA damage. ( A ) Left panel: exponentially growing wild-type cells expressing a GFP-tagged Pkc1 protein (JCY1511) were incubated for 2 h in the absence or presence of 0.2 M HU; right panel: raffinose-grown wild-type (JCY1511) and GAL1:HO (JCY1514) cells expressing a GFP-tagged Pkc1 protein were incubated for 4 h in the presence of 2% glucose or 2% galactose. Electrophoretic mobility of Pkc1-GFP was investigated by western analysis using an anti-GFP antibody. An unspecific band in the western blot or the ponceau staining of the membrane is showed as a control of migration. Activation of the checkpoint kinase Rad53 is showed as a control of DNA damage induction. ( B ) Raffinose-grown GAL1:HO (JKM139) cells were incubated for 4 h in the presence of 2% glucose or 2% galactose. Electrophoretic migration of endogenous Pkc1 was investigated by western analysis using an anti-yeast Pkc1 antibody. ( C ) Raffinose-grown GAL1:HO (JKM139) and GAL1:HO tel1 (JCY1654) cells were incubated for 4 h in the presence of 2% glucose or 2% galactose. Electrophoretic migration of endogenous Pkc1 after incubation of cell extracts in the absence or presence of <t>λ</t> <t>protein</t> <t>phosphatase</t> was analyzed as in (B). ( D ) Electrophoretic migration of Pkc1-GFP in wild-type (W303-1a) mec1 (JCY1580) and tel1 (JCY1583) cells after addition of HU was analyzed as described in (A). ( E ) Raffinose-grown GAL1:HO (JKM139) and GAL1:HO tel1 (JCY1654) cells transformed with pPKC1-GFP plasmid were incubated for 4 h in the presence of 2% glucose or 2% galactose. Electrophoretic migration of Pkc1-GFP was investigated by western analysis using an anti-GFP antibody.
Lambda Protein Phosphatase Kit K1102, supplied by ApexBio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lambda protein phosphatase kit k1102 - by Bioz Stars, 2026-08
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lambda protein phosphatase  (GeneSearch Inc)
90
GeneSearch Inc lambda protein phosphatase
Analysis of Pkc1 electrophoretic mobility after induction of DNA damage. ( A ) Left panel: exponentially growing wild-type cells expressing a GFP-tagged Pkc1 protein (JCY1511) were incubated for 2 h in the absence or presence of 0.2 M HU; right panel: raffinose-grown wild-type (JCY1511) and GAL1:HO (JCY1514) cells expressing a GFP-tagged Pkc1 protein were incubated for 4 h in the presence of 2% glucose or 2% galactose. Electrophoretic mobility of Pkc1-GFP was investigated by western analysis using an anti-GFP antibody. An unspecific band in the western blot or the ponceau staining of the membrane is showed as a control of migration. Activation of the checkpoint kinase Rad53 is showed as a control of DNA damage induction. ( B ) Raffinose-grown GAL1:HO (JKM139) cells were incubated for 4 h in the presence of 2% glucose or 2% galactose. Electrophoretic migration of endogenous Pkc1 was investigated by western analysis using an anti-yeast Pkc1 antibody. ( C ) Raffinose-grown GAL1:HO (JKM139) and GAL1:HO tel1 (JCY1654) cells were incubated for 4 h in the presence of 2% glucose or 2% galactose. Electrophoretic migration of endogenous Pkc1 after incubation of cell extracts in the absence or presence of <t>λ</t> <t>protein</t> <t>phosphatase</t> was analyzed as in (B). ( D ) Electrophoretic migration of Pkc1-GFP in wild-type (W303-1a) mec1 (JCY1580) and tel1 (JCY1583) cells after addition of HU was analyzed as described in (A). ( E ) Raffinose-grown GAL1:HO (JKM139) and GAL1:HO tel1 (JCY1654) cells transformed with pPKC1-GFP plasmid were incubated for 4 h in the presence of 2% glucose or 2% galactose. Electrophoretic migration of Pkc1-GFP was investigated by western analysis using an anti-GFP antibody.
Lambda Protein Phosphatase, supplied by GeneSearch Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/%CE%BB+protein+phosphatase/10__3233_slash_hab___2006___15302-45-10-13?v=GeneSearch+Inc
Average 90 stars, based on 1 article reviews
lambda protein phosphatase - by Bioz Stars, 2026-08
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λ protein phosphatase  (Biosynth Carbosynth)
N/A
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recombinant lambda protein phosphatase  (Creative BioMart)
N/A
Lambda-PPase was expressed as a recombinant protein in E.coli and highly purified. This product is an intact enzyme of high quality without tag.Lambda Protein Phosphatase (Lambda-PPase) is a Mn+2-dependent protein phosphatase with activity towards phosphorylated
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Image Search Results


Analysis of Pkc1 electrophoretic mobility after induction of DNA damage. ( A ) Left panel: exponentially growing wild-type cells expressing a GFP-tagged Pkc1 protein (JCY1511) were incubated for 2 h in the absence or presence of 0.2 M HU; right panel: raffinose-grown wild-type (JCY1511) and GAL1:HO (JCY1514) cells expressing a GFP-tagged Pkc1 protein were incubated for 4 h in the presence of 2% glucose or 2% galactose. Electrophoretic mobility of Pkc1-GFP was investigated by western analysis using an anti-GFP antibody. An unspecific band in the western blot or the ponceau staining of the membrane is showed as a control of migration. Activation of the checkpoint kinase Rad53 is showed as a control of DNA damage induction. ( B ) Raffinose-grown GAL1:HO (JKM139) cells were incubated for 4 h in the presence of 2% glucose or 2% galactose. Electrophoretic migration of endogenous Pkc1 was investigated by western analysis using an anti-yeast Pkc1 antibody. ( C ) Raffinose-grown GAL1:HO (JKM139) and GAL1:HO tel1 (JCY1654) cells were incubated for 4 h in the presence of 2% glucose or 2% galactose. Electrophoretic migration of endogenous Pkc1 after incubation of cell extracts in the absence or presence of λ protein phosphatase was analyzed as in (B). ( D ) Electrophoretic migration of Pkc1-GFP in wild-type (W303-1a) mec1 (JCY1580) and tel1 (JCY1583) cells after addition of HU was analyzed as described in (A). ( E ) Raffinose-grown GAL1:HO (JKM139) and GAL1:HO tel1 (JCY1654) cells transformed with pPKC1-GFP plasmid were incubated for 4 h in the presence of 2% glucose or 2% galactose. Electrophoretic migration of Pkc1-GFP was investigated by western analysis using an anti-GFP antibody.

Journal: Nucleic Acids Research

Article Title: Protein kinase C controls activation of the DNA integrity checkpoint

doi: 10.1093/nar/gku373

Figure Lengend Snippet: Analysis of Pkc1 electrophoretic mobility after induction of DNA damage. ( A ) Left panel: exponentially growing wild-type cells expressing a GFP-tagged Pkc1 protein (JCY1511) were incubated for 2 h in the absence or presence of 0.2 M HU; right panel: raffinose-grown wild-type (JCY1511) and GAL1:HO (JCY1514) cells expressing a GFP-tagged Pkc1 protein were incubated for 4 h in the presence of 2% glucose or 2% galactose. Electrophoretic mobility of Pkc1-GFP was investigated by western analysis using an anti-GFP antibody. An unspecific band in the western blot or the ponceau staining of the membrane is showed as a control of migration. Activation of the checkpoint kinase Rad53 is showed as a control of DNA damage induction. ( B ) Raffinose-grown GAL1:HO (JKM139) cells were incubated for 4 h in the presence of 2% glucose or 2% galactose. Electrophoretic migration of endogenous Pkc1 was investigated by western analysis using an anti-yeast Pkc1 antibody. ( C ) Raffinose-grown GAL1:HO (JKM139) and GAL1:HO tel1 (JCY1654) cells were incubated for 4 h in the presence of 2% glucose or 2% galactose. Electrophoretic migration of endogenous Pkc1 after incubation of cell extracts in the absence or presence of λ protein phosphatase was analyzed as in (B). ( D ) Electrophoretic migration of Pkc1-GFP in wild-type (W303-1a) mec1 (JCY1580) and tel1 (JCY1583) cells after addition of HU was analyzed as described in (A). ( E ) Raffinose-grown GAL1:HO (JKM139) and GAL1:HO tel1 (JCY1654) cells transformed with pPKC1-GFP plasmid were incubated for 4 h in the presence of 2% glucose or 2% galactose. Electrophoretic migration of Pkc1-GFP was investigated by western analysis using an anti-GFP antibody.

Article Snippet: The dephosphorylation assays were carried out by incubating 10 mg of cell extract in λ protein phosphatase buffer (50 mM Tris-HCl pH 7.8, 5 mM DTT, 2 mM MnCl2, 100 μg/ml bovine serum albumin) containing 200 U of λ protein phosphatase (MERCK Millipore) for 20 min at 30°C.

Techniques: Expressing, Incubation, Western Blot, Staining, Membrane, Control, Migration, Activation Assay, Transformation Assay, Plasmid Preparation